Nucleus reticularis thalami connections with the mediodorsal thalamic nucleus: A light and electron microscopic study in the monkey

Abstract

Wheat germ agglutinin conjugated horseradish peroxidese (WGA-HRP) and biotinylated dextran amine (BDA) were used as tracers to study nucleus reticularis (NRT) connections with the mediodorsal nucleus (MD). Injections of WGA-HRP in the MO resulted in retrograde labeling of cells in the anteromedial segment of the NRT, the so-called rostral NRT pole. Injections of WGA-HRP and BDA in this NRT region resulted in dense anterograde labeling in the MD. Labeled NRT fibers gave off several collaterals to different MD regions ending with terminal plexuses of thin varicose fibers. In the neuropil, the varicosities were distributed at random, and no tendency to form pericellular baskets was noted. Postembedding immunocytochemistry for GABA was performed on the tissue containing anterograde WGA-HRP label for identification of NRT boutons under electron microscope. The double-labeled boutons were of small to medium size, contained a large number of pleomorphic vesicles, few mitochondria, and formed multiple symmetric synaptic contacts. The number of contacts established by one bouton ranged from 1 to 4 with an average of 1.8 per bouton. About 60% of these boutons made synapses on distal dendrites of GABAergic local circuit neurons; 33% of synaptic contacts were on distal dendrites of thalamocortical neurons, and the rest on their proximal dendrites and soma. NRT boutons were also found in serial synapses and triads. The results demonstrate that the NRT input to the MD is organized so that a single fiber innervates different MD regions and its terminals form numerous synaptic contacts mostly on the distal dendrites of a large number of local circuit neurons and projection neurons.