Nucleus of the solitary tract and dorsal motor nucleus of the vagus nerve of the pigeon: localization of peptide and 5-hydroxytryptamine immunoreactive fibers.

Abstract

The distribution of peptide and serotonin fibers in the nucleus of the solitary tract (NTS) and the dorsal motor nucleus of the vagus nerve (DMNX) in the pigeon (Columba livia) was investigated immunocytochemically. This information was correlated with the viscerotopic organization of the nuclei and with central NTS circuitry to suggest the role of the neurochemical containing fibers in the regulation of organ function. The distribution of fibers containing cholecystokinin (CCK), calcitonin gene-related peptide (CGRP), enkephalin (ENK), neuropeptide Y (NPY), neurotensin (NT), substance P (SP), somatostatin (SS), vasoactive intestinal peptide (VIP), and 5-hydroxytryptamine (5-HT) was determined. Each substance had a distinct distribution within the subnuclei of NTS-DMNX, but certain generalities can be deduced. In the DMNX, fibers immunoreactive for ENK, NT, and SP were found in greatest concentration, while CGRP and 5-HT immunoreactive fibers were the least dense. This suggests that ENK, NT, and SP may have a significant modulatory effect on gastrointestinal functions. In the NTS overall, ENK, NT, SP, and VIP fibers were found in high density, CCK, NPY, SS, and 5-HT fibers were found in moderate density, and CGRP fibers were found in low density. However, some individual NTS subnuclei were found to contain moderate to high concentrations of each of the substances, including CGRP. Fibers containing CCK, ENK, NT, SP, SS, and VIP in the medial dorsal NTS subnuclei may regulate gastroesophageal functions. The caudal part of subnucleus lateralis parasolitarius did not contain most of the substances, which suggests that pulmonary function is not modulated by these neurochemicals. The boundaries of a subnucleus could sometimes be demarcated by a change in density of immunoreactive fibers between adjacent subnuclei. This was particularly evident in NTS subnuclei medialis dorsalis anterior centralis and lateralis parasolitarius, and in DMNX subnucleus posterior dorsalis magnocellularis. The selective distribution of peptide and serotonin immunoreactive fibers in various subnuclei of NTS-DMNX suggests that these substances may be differentially involved in neural circuits that mediate cardiovascular and gastrointestinal functions.