Abstract
Insulin-like growth factor binding protein-3 (IGFBP-3) gene is a structural gene associated with the growth and development of the animals. The present investigation was carried out to unravel nucleotide sequence and polymerase chain reaction- restriction fragment polymorphism (PCR-RFLP) of IGFBP-3 gene in buffalo. Genomic DNA was isolated from a total of 157 animals belonging to Murrah, Surti, Jaffarabadi and Nagpuri breeds of Indian riverine buffalo. A 655 bp of IGFBP-3 gene was amplified in all the breeds and amplicons were digested with Hae III, Taq I and Msp I restriction enzymes. On digestion with Hae III yielded single restriction pattern of 8 fragments of sizes 201, 165, 154, 56, 36, 19, 16 and 8 bp in all the animals studied. Similarly Taq I and Msp I also revealed single restriction pattern yielding fragments of sizes 240 and 415 bp and 145 and 510 bp, respectively. This shows non- polymorphic nature of restriction sites in buffalo. Nucleotide sequencing of 587 bp of IGFBP-3 gene in Murrah buffalo was done and submitted to the GenBank (Accession No. AY304829). Nucleotide sequencing revealed an addition of 4 bases in the intronic region as compared to cattle. (Asian-Aust. J. Anim. Sci. 2004. Vol 17, No. 7 : 910-913)
Highlights
Key Words : Buffalo, Insulin-like growth factor binding protein-3 (IGFBP-3), Sequencing, PCR-RFLP, Polymorphism gene have been reported in cattle (Maciulla et al, 1997; Haegeman et al, 1999; Sun et al, 2002)
IGF-I and IGF-II are a couple of hormones involved breed maintained at Gujarat Agricultural University, Anand in the process of mammalian growth and regenerative (Gujrat); 32 animals of Jaffarabadi breed maintained at processes besides having active role in mammary gland cattle breeding farm, Gujarat Agricultural University, development (Hossner et al, 1997) and has a role in Junagarh (Gujarat) and 46 animals of Nagpuri breed from muscle protein synthesis in young chickens
Nucleotide sequencing of 587 bp of IGFBP-3 gene in Murrah buffalo was done and submitted to the GenBank (Accession No AY304829) (Figure 1). This was the first report of sequencing of buffalo IGFBP-3 gene
Summary
Molecular Genetics Laboratory, Animal Genetics Division, Indian Veterinary Research Institute, Izatnagar. A 655 bp of IGFBP-3 gene was amplified in all the breeds and amplicons were digested with Hae III, Taq I and Msp I restriction enzymes. On digestion with Hae III yielded single restriction pattern of 8 fragments of sizes 201, 165, 154, 56, 36, 19, 16 and 8 bp in all the animals studied. Taq I and Msp I revealed single restriction pattern yielding fragments of sizes 240 and 415 bp and 145 and 510 bp, respectively. This shows nonpolymorphic nature of restriction sites in buffalo. Nucleotide sequencing of 587 bp of IGFBP-3 gene in Murrah buffalo was done and submitted to the GenBank (Accession No AY304829).
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