Abstract
Two circular single-stranded DNAs designated DNA I and II of the banana bunchy top virus (BBTV) genome were cloned and sequenced. The sequence of BBTV DNA I consisted of 1,106 nucleotides and contained four open reading frames (ORFs) potentially encoding proteins with molecular masses ranging from 5.4 to 33.18 kDa and one putative stemloop structure of 29 nucleotides. The sequence of BBTV DNA II had 1,096 nucleotides and contained four ORFs encoding putative proteins with sizes ranging from 5.6 to 32.77 kDa and one putative stem-loop structure of 32 nucleotides. The largest ORF (V2) in DNA II encoded a putative replicase with the nucleotide triphosphate-binding motif(GGEGKS)
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