Abstract

The DNA sequence of the resolvase gene, resolution sites, and the region between the transposition functions and the end of the mercury resistance operon of the bacterial transposon, Tn 3926, is presented. The sequence of Tn 3926 upstream of the resolution sites is homologous to that bordering the 11.2-kb insert of Tn 21, supporting the idea that this insert transposed into a progenitor of Tn 3926. This region of Tn 3926 also shows 97.0% identity to the mercury-resistant determinant of the plasmid, pKLH2, suggesting that this plasmid once harbored a close relative of Tn 3926. It is proposed that the mercury resistance operon of Tn 3926 will have a structure very similar to that found on pKLH2.

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