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Abstract
The thyP3 gene, encoding thymidylate synthetase, from the Bacillus subtilis phage φ3T has been cloned and the nucleotide sequence determined. The derived amino acid sequence indicates a subunit M r of 32748. The primary amino acid sequence is compared with the sequences of the analogous proteins specified by Escherichia coli ( thyA), Lactobacillus casei, ( thyA) and phage T4 ( td). Extensive conservation exists in all four sequences implying a shared tertiary structure.
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