Abstract
Nucleotide sequence of the metL gene of Escherichia coli. Its product, the bifunctional aspartokinase ii-homoserine dehydrogenase II, and the bifunctional product of the thrA gene, aspartokinase I-homoserine dehydrogenase I, derive from a common ancestor.
Highlights
The total nucleotide sequence (2427 nucleotides) of have previously cloned the metJBLF gene cluster in the the metL gene ofEscherichia colci oding for the dimeripclasmid pBR322 and have localized precisely the origin of aspartokinaseIl-homoserinedehydrogenase II
Nucleotide Sequence Determinations-The nucleotide sequences were determined by the chemical methodof Maxam and Gilbert [6]
Hydrogenase 11 and aspartokinase I-homoserine dehydrogen- Nucleotide Sequence of the metL Gene-A restriction fragase I possess remarkable similarities [1];when they are ment isolated from a Ah80 transducing bacteriophage by cleavcompared using immunochemical techniques, a cross-reaction age with EcoRI and PstI was cloned in pBR322 [5]
Summary
The total nucleotide sequence (2427 nucleotides) of have previously cloned the metJBLF gene cluster in the the metL gene ofEscherichia colci oding for the dimeripclasmid pBR322 and have localized precisely the origin of aspartokinaseIl-homoserinedehydrogenase II We present the amino acid residues, M, = 88,726jchain)is presented. Comparison of thetranslatedsequencewiththat of between the amino acid sequence of its product, aspartokinase aspartokinase I-homoserine dehydrogenIa,stehe prod- 11-homoserinedehydrogenase 11,and theamino acid sequence uct of the thrA gene of the sameorganism, shows that of the product of thrA, aspartokinase I-homoserine dehydrothe two proteins are highly homologous derive from a common ancestor
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