Abstract
The 12177-bp nucleotide (nt) sequence of the late region of Bacillus phage φ29 genome was determined. This sequence completes the entire 19285-bp sequence of phage φ29 DNA. Eleven open reading frames were found in this region, and these were assigned to eleven late genes. Ribosome-binding sites and a potential transcriptional promoter and terminator are considered. The nt sequence was compared to the homologous region of the closely related phage PZA and tolerated variations at the nt and amino acid (aa) level were evaluated. The most frequent changes are silent nt substitutions in the third position of codons, but aa substitutions are also found.
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