Abstract
We have cloned two phospholipase A2 (PLA2) DNA complementary to RNA that contained nucleotide sequences encoding pancreas loop by the reverse transcription-polymerase chain reaction cloning procedure using messenger RNA isolated from Laticauda semifasciata pancreas. Additionally, a gene clone encoding PLA2 with the pancreatic loop sequence was isolated from a L. semifasciata genomic library. Subsequent sequence analysis revealed that PLA2 clones encoding group IB″ PLA2. Comparative analysis of group IA and IB″ PLA2 genes revealed that the exon-intron organization is conserved in the genes of both groups. The invaded sequences in the second intron were very similar to those of the L. semifasciata group IA gene. This observation suggested that the integration of the invaded sequences occurred before the divergence of groups IA and IB″ during the evolution of PLA2 gene. The comparative analysis revealed that the arising of group IA PLA2 occurred by the deletion and substitution of nucleotide sequences in exon III region during the process of accelerated evolution.
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