Nucleotide sequence of human Gγ globin messenger RNA

Abstract

The nucleotide sequences of the entire coding portion and 3'-untranslated portion of the human Gγ globin mRNA have been determined by direct nucleotide sequence analysis of cloned human Gγ globin cDNAs. These nucleotide sequences have been compared to the previously published sequences of the human β globin mRNA and Aγ globin mRNA. No differences were detected between the coding sequences of Gγ and Aγ globin mRNAs except for the expected difference at codon No. 136, but a number of differences were detected in the 3'-untranslated sequences of the two mRNAs. Codon usage of Gγ globin mRNA, although similar to that of the related β globin mRNA, displays a few notable differences such as the use of UUG for leucine and GGA for glycine that are not utilized in β glob in mRNA.