Abstract
7 S RNA, a component of normal higher eukaryotic cells and several oncornaviruses, was shown to be conserved in evolution (Erikson, E., Erikson, R. L., Henry, B., and Pace, N. R. (1973) Virology 53, 40-46). Recently, 7 S RNA was shown to be partially complementary to Alu family DNA sequences (Weiner, A. (1980) Cell 22, 209-218). In the present study the nucleotide sequence of Novikoff hepatoma 7 S RNA was determined to be: (formula, see text) Comparison of 7 S RNA, Alu and B1 family DNA, and La 4.5 S RNA sequences for homologies showed that 1) one-third of 7 S RNA, mainly the 5'-end, was homologous to Alu and B1 family sequences; 2) one 300-nucleotide long Alu family sequence contained two binding sites for 7 S RNA; and 3) the 5'-ends of 7 S RNA and La 4.5 S RNA also had extensive (60%) homologies. A model for the secondary structure of 7 S RNA based on maximal base pairing and preferential nuclease cleavage sites is also presented.
Highlights
S RNA, a component of normal higher eukaryotic cells and several oncornaviruses, was shownto be conserved in evolution
Oligonucleotide T-16had the nucleotide composition C,U,A,G when digestedwith T2 RNase and C2,A,G when digestedwith nuclease P1, showingthat Up was the 5"terminus. These dataalong with the dataobtained with RNase A digestion showedthe sequence was U-C-C-A-Gpfor oligonucleotideT-16
The 7 S RNA was of interest because it is encoded by the host genome and is incorporated into several oncornaviruses [1,2,3]
Summary
S RNA, a component of normal higher eukaryotic cells and several oncornaviruses, was shownto be conserved in evolution 7 S RNA was shown to be partially complementary to Alu family DNA sequences In the present study the nucleotide sequence of Novikoff hepatoma 7 S RNA was determined to be: pppGCCGGGCGGU GCGCACGCCU GUAGUCCCAG. S RNA was first identified as a component of some oncornaviruses such as Rous sarcoma, avianmyeloblastosis, mouse sarcoma, and L cell virions [1]. 7 S RNA was shown to be present in uninfected cells; fingerprint analysis showed the 7 S RNA of several oncornaviruses anudninfected cells was very similar [2, 3]. Two studies[7,8]found 7 S RNA in polyribosomes, but this point has been controversi(a4l)
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