Abstract
The nucleotide sequence of a 3·2 kb region following the hydrogenase structural operon ( hupSLCDEF) in the H 2-uptake gene cluster from Rhizobium leguminosarum bv viciae strain 128C53 has been determined. Five closely linked genes encoding products of 16·3 (HupG), 30·5 (HupH), 8·0 (HupI), 18·4 (HupJ) and 38·7 (HupK) kDa were identified 166 bp downstream from hupF. Transposon insertions into hupG, hupH, hupJ and hupK suppress the H 2-oxidizing capability of the wild-type strain. The amino acid sequence deduced from hupI contains two Cys-X-X-Cys motifs, characteristic of rubredoxins, separated by 29 amino acid residues showing strong sequence homology with other bacterial rubredoxins. The amino acid-derived sequence from hupG and hupH showed homology to products from genes hyaE and hyaF of the operon encoding hydrogenase 1 from Escherichia coli, and hupJ and hupK were related to open reading frames identified in Rhodobacter capsulatus and Azotobacter vinelandii hydrogenase gene clusters. An involvement of the hupGHTJK gene cluster in redox reactions related to hydrogenase synthesis or activity is predicted on the basis of the function as electron carrier attributed to rubredoxin.
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