Nucleotide sequence analysis of 95 kb near the 3′ end of the murine T-cell receptor [formula omitted] chain locus: Strategy and methodology

Abstract

The nucleotide sequence of a region at the 3′ terminus of the murine T-cell receptor α δ chain locus is presented. This region, which encodes the constant region genes for α and δ chain polypeptides and all 50 joining gene segments for the α chain polypeptide, spans 94,647 bp and includes more than 50 noncoding sequence elements important for T-cell receptor gene rearrangement and expression. DNA sequencing of this region included complete analysis of two cosmid clones and five additional restriction fragments using a random subcloning approach with various manual and automated sequencing strategies. The automated sequencing strategies hold considerable promise for future large-scale DNA sequencing efforts.