Abstract
Characidae is the largest and more diversified family from Characiformes and presents several classification problems, with several genera currently allocated as incertae sedis, such as the genus Hemigrammus. The upper Parana river floodplain is an environment with high fish diversity. There is at least one species of Hemigrammus, however there are divergences among some authors about the number and the identification of the species from this genus. Therefore the goal of this study was to characterize, using a molecular approach, individuals of Hemigrammus from the upper Parana river floodplain and to compare them with individuals from the type locality of Hemigrammus marginatus, since this is the only species distributed in this floodplain. For this, the DNA was extracted and a partial region from the mitochondrial genes ATPase 6 and ATPase 8 were amplified and sequenced. The results evidenced the existence of two species of Hemigrammus in the floodplain, although impossible to be distinguished only through morphological traits. High nucleotide diversity among individuals from the upper Parana river in relation to those from the type locality was also observed, indicating that both species of Hemigrammus present in the upper Parana river floodplain are not Hemigrammus marginatus.
Highlights
The order Characiformes is one of the largest group of freshwater fish worldwide including 18 families, about 270 genera and at least 1,674 species (NELSON, 2006)
The genera incertae sedis within Characidae comprise about 620 species, 43 from the genus Hemigrammus, Gill, 1858 (LIMA et al, 2003)
The amplification of the partial sequence of the genes ATPase 6 and ATPase 8 produced a fragment with approximately 1,000 base pairs
Summary
The order Characiformes is one of the largest group of freshwater fish worldwide including 18 families, about 270 genera and at least 1,674 species (NELSON, 2006). These organisms present a wide diversity of forms, inhabiting in various types of aquatic environments. Characidae is the largest and more diversified family, with 12 subfamilies, 16 genera and more than 962 species (NELSON, 2006). The genera incertae sedis within Characidae comprise about 620 species, 43 from the genus Hemigrammus, Gill, 1858 (LIMA et al, 2003)
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