Abstract
M O N D A Y 615 Nucleotide Changes in Hypervariable Regions of Genes Coding Heavy Chains of IgE in Patients with Atopic Rhinitis, Bronchial Asthma and Atopic Dermatitis L. P. Titov, E. A. Stolyarova, T. A. Stolyarova, L. M. DuBuske; Republican Scientific and Practical Center for Epidemiology and Microbiology, Minsk, BELARUS, Immunology Research Institute of New England, Gardner, MA. RATIONALE: Prevalence of allergic diseases has increased in Belarus associated with increased production of IgE. METHODS: Sequences of IgE VH genes from databases of Gene-Bank patients with bronchial asthma (506), atopic rhinitis (133), dermatitis (83) and sequences of IgE VH genes from non-atopic donor (127) were studied. For identification of gene families, the software IMGT-QEST was used. Nucleotides replacement was performed by VVK 3.4 programs. RESULTS: VH genes coding IgE antibodies in non-atopic donors are mainly VH3 family (42; 86%), with VH4 and VH5 families also included in the IgE synthesis (17; 29% and 16; 54%, respectively). In patients with asthma versus allergic rhinitis, genes coding IgE mostly were formed by VH3 family (50; 59% versus 42; 86 %, respectively) with less from the VH 5 family (17; 39% versus 16; 54%) and VH4 family (12; 06% versus 17, 29%, respectively). VH genes coding of IgE in atopic dermatitis patients are based on VH4, VH5 and VH3 families (31; 32% versus 20; 48% and versus 24; 1% respectively).The lowest GC content was in the VH3 family (0.54360.002) with VH4 and VH5 families having 0.57260.002 and 0.57560 0019, respectively. VH3 family genes from asthmatics are characterized by predominance of change AT/GC over the substitution GC/AT (p<0.05). CONCLUSIONS: Nucleotide changes in hypervariable regions of genes coding heavy chains of IgE in patients with atopic rhinitis, bronchial asthma and atopic dermatitis differ and may be related differences in expression of these allergic diseases.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
