Abstract
A frequently used method for the analysis of single nucleotide variations in DNA is allele-specific PCR (asPCR). AsPCR poses major challenges to the DNA polymerase used for this purpose e. g., the avoidance of the formation of false results due to the formation of side products. Several independently conducted studies show that a highly discriminative mutant of Taq DNA polymerase is ideally suited for use in asPCR in numerous research areas ranging from mutation detection to genome editing.
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