Nucleoside-Nucleotide-Free Diet Suppresses Cytokine Production and Contact Sensitivity Responses in Rats With Trinitrobenzene Sulphonic Acid-Induced Colitis

Abstract

AbstractWe examined the effects of dietary nucleoside-nucleotide mixture on synthesis of inflammatory cytokines, interleukin-8 and tumor necrosis factor-a, in sensitized and nonsensitized colitic rats. Sensitized and nonsensitized colitic rats that were fed a nucleosidenucleotide mixture had greater colonic weight and macroscopic and microscopic damage scores than nucleoside-nucleotide-free sensitized and nonsensitized colitic rats. Increased colonic tumor necrosis factor-a and interleukin- 8 concentrations were associated with increased colonic inflammation and ulceration in the nucleoside-nucleotide mixture-fed group. There was also increased ear thickness in the nucleoside-nucleotide mixture-fed sensitized and nonsensitized colitic rats, which correlated highly with increased tumor necrosis factor-a and interleukin-8 levels in the ear lobes. Nucleoside-nucleotide-free diets may suppress cytokine secretion, thereby reducing colonic damage and contact sensitivity responses in colitic rats.