Abstract
RT-PCR of RNA isolated from monolayers of the human colonic epithelial cell lines T84 and Caco-2 demonstrated the presence of mRNA for the two cloned Na +-independent equilibrative nucleoside transporters, ENT1 and ENT2, but not for the cloned Na +-dependent concentrative nucleoside transporters, CNT1 and CNT2. Uptake of [ 3H]uridine by cell monolayers in balanced Na +-containing and Na +-free media confirmed the presence of only Na +-independent nucleoside transport mechanisms. This uptake was decreased by 70–75% in the presence of 1 μM nitrobenzylthioinosine, a concentration that completely inhibits ENT1, and was completely blocked by the addition of 10 μM dipyridamole, a concentration that inhibits both ENT1 and ENT2. These findings indicate the presence in T84 and Caco-2 cells of two functional Na +-independent equilibrative nucleoside transporters, ENT1 and ENT2.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Biomembranes
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