Abstract
Digestion of chromatin in erythrocyte nuclei under a variety of conditions leads to the following conclusions:(1)The size of DNA in the nuclease-resistant core particles in chromatin is 140 base pairs. This resistant DNA is associated with an average of two each of the “inner” histones, H2A, H2B, H3 and H4 to form ∼200, 000 daltons core particles.(2)These core particles are separated by spacer regions averaging 40 base pairs of DNA in chicken erythrocytes. The size may be different in other chromatins. There appears to be some heterogeneity in the spacer length of DNA between particles.(3)The sum of the 140 base pairs DNA in the core particle plus the 40 base pairs average spacer DNA yields 180 base pairs for the repeating unit of chicken erythrocyte chromatin. This is comparable to the ∼200 base pair-subunit repeat reported for other mammalian chromatins.(4)The “outer” hi stone H1 and H5 and non-histone proteins are associated predominantly with spacer regions of DNA which connect two core particles.
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