Year-end Sale % OFF 
Abstract
Stimulation of human lymphocytes with phytohaemagglutinin in vitro induces marked changes in the properties of the nuclear deoxyribonucleoprotein complexes. These changes manifest themselves in an increased capacity to bind acridine orange, acetylation of histories and an altered affinity for actinomycin D and can be observed before RNA synthesis is initiated. An increased capacity to bind acridine orange is observed in all cells but only part of the lymphocyte population appears to acetylate histones and to show an enhanced actinomycin binding. Double emulsion autoradiography after labelling with both 14C-acetate and 3H-uridine suggests that it is those cells which acetylate histone which will also initiate RNA synthesis. It is suggested that the phenomena studied mainly reflect different stages of dissociation of the nucleoprotein complex and are part of a multistep preparatory mechanism for initiation of transcription.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
