Abstract
Most of the current research on synthetic bilayer membrane-nucleic acid complexes (lipoplexes) is addressed to lipid self-assemblies as nonviral vectors for genetic material. The vast majority of synthetic nonviral vectors so far considered consists of cationic lipid assemblies where DNA binds to cationic liposomes, thanks to charge compensation between the negative polyelectrolyte and the headgroups of the cationic lipid assembly. We report a SAXS investigation with the first evidence of formation of an ordered lamellar phase of the type Lαc without any mediation from divalent cations or the presence of cationic lipids, where the complementary polynucleotide strands spontaneously order in between a lamellar fluid phase formed by the anionic 1-palmitoyl-2-oleoyl-phosphatidyl-adenosine nucleolipid, POPA. This nucleolipid has full biological compatibility and can be enzymatically cleaved by phospholipases once in living organisms. While for conventional lipoplexes the driving force leading to condensation i...
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