Abstract

Nucleolar activity and competition (amphiplasty) has been analysed in diploid, tetraploid and hexaploid species of Aegilops using a highly reproducible silver-staining procedure. A comparative analysis of somatic metaphase chromosomes by phase contrast, C-banding and Ag-staining was made to analyse the activity of the nucleolar organiser regions (NORs). With the exception of Ae. crassa 4x, amphiplasty occurs in all the tetraploid and hexaploid species analysed. The U genome from Ae. umbellulata suppresses completely the NOR activity of the genomes M° (Ae. ovata), Sv (Ae. variabilis), D (Ae. juvenalis) and C (Ae. triuncialis) and that of one pair of the nucleolar organiser chromosomes of the genomes Mc (Ae. columnaris), Mb (Ae. biuncialis), Mj (Ae. juvenalis) and Mt (Ae. triaristata 6×). The nucleolar activity of the D genome is completely suppressed by the genomes U (Ae. umbellulata), C (Ae. cylindrica) and Mv (Ae. ventricosa).

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