Abstract
Nucleoids isolated from heterogeneous populations of Caulobacter crescentus were invariably associated with the cell envelope. The cell types from which nucleoids were derived were easily identifiable because of the distinctive dimorphic cell cycle of this organism. Sucrose density gradient centrifugation of an exponentially growing culture, gently lysed at 10 °C, gave two classes of envelope-associated nucleoids. One, a broad slow sedimenting band with a sedimentation coefficient of 5600S, was comprised primarily of nucleoids from stalked cells. The other, a tight fast sedimenting band with a sedimentation coefficient of 7100S, was comprised of nucleoids from flagellate and pre-divisional cells. The DNA packing and nucleoid morphology of each class of nucleoids was examined by electron microscopy.
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