Abstract
Nucleoids, prepared by salt extraction of non-DNase-digested nuclei, have properties similar, but not identical to those of nuclear matrices which are prepared by salt extraction of DNase-digested nuclei. Nuclear matrices, retained less pulse-labelled DNA, slightly less bound DNA polyesterase α and DNA primase, but had greater in vitro DNA synthesis and in vitro priming. Nucleoids contains larger (110 S) DNA chains than nuclear matrices (30 S). Each type of residual nuclear structure could synthesize 4.5 S Okazaki fragments. When extracted with increasing concentrations of salt, DNase-digested nucleo lost the ability for further elonation of the 4.5 S DNA intermediate after 0.1–0.2 M NaCl, whereas undigested nucleo retained this ability up to 0.9 M NaCl. Chain elongation to 28 S DNA chains could be restored to nucleoids, but not to nuclear matrices, by the addition of nuclear extracts.
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