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Abstract
Here we describe some properties of nucleic acids, which observed in ‘atypical” experiments. So, we show that physical and chemical properties of nucleic acids depend on dissolved gases. We show particularly that thermal hyperchromism of DNA depends on dissolved oxygen and (or) air. It has been showed most importantly the thermal hyperchromism does not observe for DNA degassed solutions. Here we also demonstrate the difference between some chemical properties of nucleic acids in degassed solutions and in solutions saturated with hydrogen or oxygen. We illustrate too the difference between physical and chemical properties of nucleic acids in the homogenous and gradient-containing solutions; the last case we offer as approximation of the conditions, in which the nucleic acids exist in vivo.
Highlights
In most cases don't pay attention to the presence of gases in the solutions of nucleic acids (NA)
We found that heating the DNA degassed solutions does not alter their absorption in the range 220 – 300 nm
It was found that heating the saturated with oxygen DNA solutions follows a noticeable UV hyperchromism: 100 – 150 %
Summary
In most cases don't pay attention to the presence of gases in the solutions of nucleic acids (NA). The effect of dissolved air (and, obviously, oxygen) on the thermal stability of DNA in the melting experiments is mostly ignored. Need to taken into account that intestinal gases contain ~ 75% hydrogen and, as rule, don’t contain oxygen. For these reasons, we finished that the influence dissolved gases on the physical and chemical properties of NA need to study. The most part the physical and chemical properties of NA in the homogenous solutions are researching, in [1,14] It is not good enough, because in vivo NA exist mainly in the gradient-containing systems. The concentration of Na+-ions in the nuclei of somatic cells mammalians 10 times more than in cytoplasm, in [15,16,17,18,19]; in vivo DNA and RNA of mammalians exist in the systems containing two phases with different concentrations of Na+ions
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