Nucleic acids and growth of larval and early juvenile spider crab,Hyas araneus

Abstract

The spider crabHyas araneus (L.) was collected from the North Sea in winter 1986–1987 and reared in the laboratory from hatching of the Zoea I (ZI) through the first juvenile instar (CI). Within a given moult cycle, individuals of the same age were sampled in intervals of 2 (ZI, ZII, CI) or 3 d (megalopa) for analysis of dry weight (W), carbon (C), nitrogen (N), hydrogen (H), protein, DNA, and RNA. Lipid was calculated from C. Biomass, growth rate and nucleic acid contents showed high variability during each moult cycle and between instars. Instantaneous growth rates of C were high in postmoult and intermoult, and low in the premoult period of each moult cycle. A shift was observed from high rates of lipid accumulation in the postmoult and intermoult stages to proportionally increasing protein accumulation during late premoult (ZI), or throughout a major part of the remaining moult cycle (in all other instars). DNA was accumulated throughout the ZI and ZII instars, but decreased in late premoult megalopa. It increased again from late intermoult through intermediate premoult in juveniles. RNA increased continuously during ZI and ZII, and decreased in the megalopa, almost to levels that had been found immediately after hatching. In juveniles, variation in RNA followed closely those in DNA. Cell multiplication (expressed by DNA increase) dominated over increase in cell size (defined by the C/DNA ratio) during the zoeal instars and in postmoult through early intermoult in the megalopa and CI. When specific (C-related) RNA values and RNA/DNA ratios were compared with instantaneous growth rates in C and N, no general correspondence was detected. The only significant relationship between specific RNA values and instantaneous C or N growth rates was found in the megalopa. The same held for the relationship between the RNA/DNA ratio and growth. Here, in addition to the megalopa, a correspondence with C growth was also found in the CI instar. Our results suggest that variation in nucleic acids may provide useful insights into mechanisms of growth on the cellular level (cell multiplication vs cell enlargement). However, lack of general correlation with variation in growth rates ofH. araneus larvae shows that the use of nucleic acids as a measure of growth is probably based upon too simplistic assumptions; it may not yield reliable predictions, when growth is associated with developmental events.