Nucleic acid purification on a Lab-on-a-Disc with time-controlled incubation

Abstract

Here we present integrated Lab-on-a-Disc (LoaD) cartridges which are applied to the purification of nucleic acid using the silica bead based method. We utilize a novel combination of ‘event-triggered’ dissolvable film (DF) valving [1] and a centrifugo-pneumatic siphon valve (CPSV) [2] to permit timing of sample incubations, washes and DNA elution. We present two systems; one in with a conventional ‘wash through’ elution which achieves a purification yield of 32.3% ± 5.4% (n = 4). The second system, which uses a CPSV to enable extended wash and elution steps, provides an increased efficiency of 58.4 ± 7.5% (n = 3).