Abstract
NUCLEIC ACID COMPOSITION OF HUMAN LIVER CELL FRACTIONS
Highlights
The recovery during the hydrolysis and ion exchange chromatography steps was estimated by comparing the total RNA content of a given cell fraction with the sum of the nucleotides recovered from the ion exchange column
The nucleotides were estimated from the optical densities and the extinction coefficients of the nucleotides at 260 mu [14, 15]
Human adult and fetal and rat adult livers were homogenized and differentially centrifuged to obtain the various cellular fractions. These were fractionated into lipide, acid-soluble, ribonucleic acid, deoxyribonucleic acid, and protein moieties
Summary
The tissues studied included human fetal liver, obtained at t’herapeutic interruptions of pregnancy, adult human liver, obtained from normal livers during laparotomies, and adult rat liver from Wistar strain rats. King t Present address, Department of Biology, setts Institute of Technology. American Cancer of Biochemistry, Soriety and Massachu-. The suspension was separated by the differential centrifugation procedure of Hogeboom and Schneider [8] into nuclei, mitochondria, microsomes, and supernatant fractions. Microscopic examination revealed that the nuclear fraction contained a few mitochondria and some erythrocytes but no unbroken liver cells, that the mitochondrial fraction consisted of free and a few agglutinated mitochondria but no nuclei, and that the microsome and supernatant fractions were free of visible contaminants
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
