Abstract
Classical methods for isolation and fractionation of cell nuclei have been improvised with the intent to simplify and increase efficiency of fowl erythrocyte nuclear characterization. A comparative study on the ratios of the major macromolecules (DNA, RNA, and nucleoproteins) of avian erythrocyte nuclei has also been undertaken.Five avian species, Gallus domesticus (chicken), Alectoris graeca chukar (Indian partridge), Coturnix coturnix japonica (quail), Phasianus colchicus torquatus (Ring-necked pheasant) and Meleagris gallopava (Beltsville Small White turkey) were the subjects of this study.Nuclei percentage composition of DNA, RNA, and protein were determined spectrophotometrically. Ratios of these macromolecules were subsequently calculated. DNA to protein to RNA were approximately (0.9–1.0 to 0.2–0.3), respectively. The ratio of DNA to protein does not appear to be species specific. In contrast, DNA to RNA does exhibit species specificity.
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