Abstract
The ventral prostate nuclei from normal and 3-day castrated rats were subjected to mild micrococcal nuclease digestion. The prostatic binding protein (PBP) gene sequences in the nuclease-sensitive and -resistant DNAs were evaluated by hybridization with DNA probe complementary to PBP-mRNA. The reassociation kinetics showed that the nuclease-sensitive DNA from normal, but not castrated, prostate nuclei was enriched 5.6-fold in the PBP-coding sequences. Digestion of similar nuclei preparations with DNase I to 27% acid-soluble showed no preferential digestion of PBP gene sequences in normal, as compared to castrated, prostate nuclei. Analyses of the levels of PBP-mRNA sequences in nuclear and polysomal poly(A)RNAs following castration showed a drastic reduction of nuclear PBP coding sequences at a rate much greater than that of reduction of polysomal PBP-mRNA. These results support that testosterone regulates PBP gene activities at the transcriptional level by altering the conformation of prostate chromatin.
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