Abstract
The nuclease reaction of a copper complex with the macrocyclic Schiff base ligand tetrabenzo [ b, f, j, n][1,3,9,13] tetraazacyclohexadecine (TAAB) at the cleavage of DNA templates has been investigated and compared with that of the copper phenanthroline complex. The labeled single-stranded GC containing 25-mer oligonucleotide, single-stranded AT 20-mer oligonucleotide and double-stranded AT 20-mer oligonucleotide with the complemented sequence in the presence of ascorbic acid and aerobic conditions were used as the substrates. The AT specificity of Cu(TAAB) 2+ for both the single-stranded and double-stranded templates may be explained by the specific formation of intercalated complex and oxygen radicals that nick the DNA.
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