Abstract
Nuclear transfer (NT), also termed nuclear replacement or nuclear transplantation, is the method of choice for animal cloning. NT is a complex technique, which involves the removal/destruction of the nuclear DNA from an oocyte or zygote (enucleation), and replacing it with nuclear material from an embryonic, germ, or somatic cell. The newly introduced DNA interacts with the recipient cytoplasmic environment and, following complex, little known changes in the nuclear DNA, can direct development into adult fertile animals. Compared to other assisted reproductive technologies, the NT process is relatively inefficient. Nonetheless, NT is an important procedure as it allows the study of fundamental questions on epigenetic “reprogramming.” As a practical tool, NT can be used for agricultural and medical applications, especially when combined with transgenic and pluripotent stem cell technologies. Keywords: Animal cloning; Cloning efficiency; Cytoplast; Embryonic stem (ES) cells; Epigenetic; Induced pluripotent stem (iPS) cells; Mitochondrial heteroplasmy; Nuclear reprogramming; Nuclear transfer (NT); Serial NT; Somatic stem cells; Tetraploid embryo complementation; Telomere; Therapeutic cloning; Reproductive cloning; Xenotransplantation
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
