Abstract
A deliver system based on a PZLL and PAMAM block copolymer (PZLL-D3) with TAT modified on the surface has been developed. It is designed to perform highly efficient nucleus-targeted gene and drug co-delivery. Confocal laser scanning microscopy (CLSM) revealed that more TAT-PZLL-D3 entered the nucleus than does PZLL-D3 alone. The TAT-modified vector serves as a gene and drug co-delivery system to achieve high gene transfection efficiency, high apoptosis and low viability in A549 cells.
Highlights
Small cell lung cancer (SCLC) originated from bronchial neuroendocrine cells and belongs to a special type of bronchogenic carcinoma
In this study, based on transactivator of transcription (TAT)’s active transport property to the nucleus, we developed a high-efficiency nucleus-targeted co-delivery vector that delivers genes and drugs directly into the nucleus of A549 cells
It is possible to solve the problem of drug resistance of SCLC if DOX can be directly transferred into the nucleus by vector
Summary
Small cell lung cancer (SCLC) originated from bronchial neuroendocrine cells and belongs to a special type of bronchogenic carcinoma. Breaking through the barriers of chemotherapy resistance of SCLC patients and improving the effect of chemotherapy is a common concern in the field of treatment at this stage. It can be detected in SCLC tissues and SCLC cell lines cultured in vitro that the high expression of P-glycoprotein. It is well known that TAT peptides, derived from the transactivator of transcription of human immunodeficiency virus type 1 (HIV-1), are the most frequently used cell nucleus-targeting peptides [6] Because it can be recognized by the nuclear pore complex, the carrier modified by Tat can transport all kinds of goods to the nucleus accurately [7,8,9,10,11,12,13].
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