Nuclear protein synthesis in hamster kidney cells: the effect of pseudorabies virus infection.

Abstract

Incorporation of [35S] methionine into the nuclei of BHK21-C13 cells after infection with pseudorabies virus indicated that acidextractable protein synthesis diminished rapidly while salt- and urea-extractable protein synthesis remained constant for up to 3 hours after infection. The proteins synthesized and extracted under the above conditions in both exponentially-growing and serum-depleted cells were analyzed by polyacrylamide gel electrophoresis. The major qualitative changes in nuclear protein synthesis resulting from pseudorabies infection are discussed in relation to possible function.