Abstract
The quantity of virus produced per larva in Heliothis armigera larvae dosed at specified ages with a Heliothis nuclear polyhedrosis virus was determined in terms of both numbers of polyhedra and virus infectivity for H. armigera, estimated by bioassay. The virus yield increased exponentially with age of larva at dosing in the age range 0 to 6 days, the overall increase being approximately 100-fold. However, there was then a reduction in yield recorded for larvae dosed at 7 days. Additionally, the infectivity per polyhedron was significantly less than that of polyhedra from larvae dosed at lesser ages ( P < 0.05). The ratio of virus produced to the corresponding dose to infect (LD 50) was at a maximum for larvae at hatching (0 day), further confirming the desirability of directing virus applications against newly hatched larvae.
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