Abstract
Partial least squares discriminant analysis (PLS-DA) models developed from 1 H NMR spectra acquired from aqueous extracts Vitis vinifera leaf material successfully discriminated between grape phylloxera-infested and uninfested grapevines under field conditions. Loadings plots demonstrated differences in the metabolic profile between treatments in the aromatic, carbohydrate and amino acid fingerprint regions of the 1 H NMR spectrum. Resonances consistent with sucrose and the phenolic moeities caffeic acid and quercetin were up-regulated in infested grapevines, while α- and β- anomers of glucose and leucine were down regulated in infested grapevines. The detection of putative quercetin resonances corroborated with the results from a previous LC/MS study. This may lead to its inclusion as a potential candidate biomarker of grape phylloxera infestation once further validation trials have been undertaken.
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