Abstract
This study describes a mechanism by which antiestrogen-suppressed MCF-7 human breast cancer cells in long term culture are rescued by estradiol. In these cells, nafoxidine (1 μM) is a potent inhibitor of cell growth, and unlike estradiol, nafoxidine fails to induce progesterone receptors at any dose tested. If in suppressed cells, nafoxidine-containing medium is replaced by medium containing estradiol (10 nM), cell growth resumes (as measured by increases in total protein and DNA), and progesterone receptor is synthesized. The average rate of progesterone receptor induction in cells treated with estradiol after nafoxidine suppression is 7-fold greater than that of cells which were given estradiol after growth in hormone-free medium. The cells rescued by estradiol from antiestrogen suppression have a 3-fold greater progesterone receptor content at the end of the study than do those cells which received estradiol without nafoxidine pretreatment. The lag period normally seen before progesterone receptor leve...
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