Abstract
Nuclear envelopes were isolated from pea plumules by sonication of a highly purified nuclear fraction. The nuclear envelope fraction, examined in the electron microscope by both negative staining and thin sectioning techniques, was found to consists of nuclear envelope fragments of widely varying sizes. Nuclear pore complex constituents frequently could be recognized. The chemical composition of the nuclear envelope fraction was determined and compared with that of the nuclear fraction. Significant amounts of DNA and RNA were found to remain associated with the nuclear envelope fraction. It was found that the specific activities of ATPase, 5′-nucleotidase and glucose-6-phosphatase were higher in the nuclear envelope fraction than in the total nuclei.
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