Nuclear DNA and RNA amounts in the wild and cultivated urd and mung beans and their M1 plants.

Abstract

The 2C amounts of DNA for Vigna sublobata (Roxb.) Babu and Sharma (the wild relative), V. radiada (L.) Wilczek cv. PS 16 (cultivar) and V. mungo (L.) Hepper cv. T 9 (cultivar) and their M1 generation plants were determined by cytophotometric and UV spectrophotometric methods. The RNA content was determined by UV spectrophotometric methods. The range of variation of DNA per cell as determined by cytophotometry was 3.11-3.61 pg; by UV spectrophotometry it ranged from 3.77 to 4.47 pg. The RNA amount ranged from 14.00 to 17.23 pg. The wild relative showed lower DNA and RNA values. Nuclear volume was lower in the wild relative than the cultigens. These results indicate that the evolution is towards an increase in nuclear volume, 2C DNA and RNA values. Deletions and translocations seem to have played a great role in genome evolution.