Abstract
Nuclear and mitochondrial DNA synthesis during sporulation of Saccharomyces cerevisiae has been studied in a wild-type ( aα) strain and 3 sporulation deficient strains. We find that in a strain carrying a dominant mutation which prevents sporulation, nuclear DNA synthesis is initiated but not completed; mitochondrial DNA synthesis, on the other hand, does take place. In aa and αα diploids no initiation of nuclear DNA synthesis is seen to occur, and only a very low level of mitochondrial DNA synthesis is observed. We conclude that mitochondrial DNA synthesis in sporulation medium is uncoupled from nuclear DNA synthesis. In addition, the steps at which the sporulation process is arrested in aa and αα cells and in the dominant mutant can be ordered in time as being before and after the initiation of nuclear DNA synthesis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
