Nuances of Erlich Ascites Carcinoma Cells Growth and how the External Electrostatic Field Affect Ascites Tumor Growth

Abstract

In the present work, the Erlich Ascites Carcinoma (EAC) cells in vitro growth kinetics, and its correlation with surveillance of EAC-infected mice is reported. EAC cells as well as EAC-inoculated mice have been exposed to the external Electrostatic Field (ESF). The kinetics of EAC cells growth in vitro was characterized with classical periods: lag-phase-0-3 days; exponential phase-3-12 days; stationary phase- 12-16 days; decline phase-16-24 days of cultivation. However, for the first time, during 24 days cultivation, drops of cell concentration had been detected: at 7th, 14th, and 21st days. The observed phenomenon had not been described earlier for in vitro conditions, but resembles the in vivo observation shown in literature. Analysis of correlation between in vitro growth of cells and in vivo surveillance of mice revealed their inverse character at 7-15 days. Hence, the correlation between EAC cells growth in vitro and in vivo was shown. This may be a substantial contribution to the knowledge of tumor development. Above it, EAC infected mice exposed to the ESF shown diminished surveillance at the days of exposure and enhanced surveillance after stopping the ESF action. It has been shown that ESF cause an EAC cell damage as well as host leucocyte cell is. But unlike to leucocytes, EAC cells rapidly accelerate growing after the end of the exposure. So, finally ESF favors tumor growing with simultaneous inhibition of host immune defense.