Abstract
BackgroundSLC10A1 gene codes NTCP, a receptor through which the hepatitis B virus (HBV) gets access into hepatocytes - a stage of the viral cycle necessary for replication. Polymorphism variants of SLC10A1 play roles in HBV infection, viral clearance, treatment outcome, and complications, in diverse ethnic groups and countries. However, no such study has been conducted in the Ghanaian population, a country with HBV endemicity. Therefore, an exploratory study was conducted to investigate the presence of three (3) single nucleotide polymorphisms (SNPs) in the SLC10A1 gene (rs2296651, rs61745930, and rs4646287) and assessed the risk of HBV infection among the Ghanaian population.MethodPolymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the presence of the SNPs among 292 participants comprising 146 HBV infected persons as case-subjects and 146 HBV non-infected persons as control-subjects.ResultsThe minor allele frequency (T) of rs2296651 was present in a significantly high proportion of cases compared with the control group (11.6% vs. 3.1%, p < 0.0001). The homozygote recessive variant of rs61745930 was present in 2.7% of the control group and 5.5% of the case group. Moreover, the minor allele frequencies of rs4646287 were 9.3 and 8.2% among the control and the case group, respectively (p = 0.767). Under the dominant (CC) genetic model of inheritance, rs2296651 was found to be protective of HBV infection [OR = 0.18 (0.07–0.44)], whereas under the co-dominant and additive model, rs2296651 was a potential risk factor for HBV infection [OR = 5.2 (95%CI: 2.1–12.8); 3.5 (95%CI: 1.6–7.6], respectively. Variants of rs61745930 and rs4646287 were not associated with HBV infection (p > 0.05). Polymorphisms in SLC10A1, however, did not show any significant association with HBV infectivity (p > 0.05).ConclusionThe study highlights some polymorphism proof that variants rs2296651, rs61745930, and rs4646287 exist in HBV-infected individuals in Ghana. Although variant rs2296651 was found to be associated with HBV infection, this association warrants more studies. Polymorphisms in SLC10A1 were not associated with HBV infectivity among the Ghanaian population. Further investigation is warranted to assess the offensive role of the relationship between rs2296651 and HBV infectivity.
Highlights
The prevalence of hepatitis B virus (HBV) infection in regions of high endemicity ranges from 6 to 8%; in Africa, prevalence rates of 10–15% have been reported [1, 2]
The study highlights some polymorphism proof that variants rs2296651, rs61745930, and rs4646287 exist in HBV-infected individuals in Ghana
Variant rs2296651 was found to be associated with HBV infection, this association warrants more studies
Summary
The prevalence of hepatitis B virus (HBV) infection in regions of high endemicity ranges from 6 to 8%; in Africa, prevalence rates of 10–15% have been reported [1, 2]. Within the adult population in Ghana, prevalence rates between 10 and 13% have been recorded across the ten regions with a national average rate of 12.3% [3], which is about 50% higher than the current global average This predisposes at least 1/10th of every Ghanaian adult to the risk of later suffering or even dying from HBV- associated complications such as cirrhosis or Hepatocellular carcinoma (HCC) [4, 5]. Solute carrier family 10 A1 (SLC10A1) gene-coded Na+-taurocholate cotransporting polypeptide (NTCP) is another molecule that influences the outcome of HBV infection It is a protein receptor through which the HBV gets access into the hepatocytes, a stage of the viral cycle necessary for replication [8, 9]. An exploratory study was conducted to investigate the presence of three (3) single nucleotide polymorphisms (SNPs) in the SLC10A1 gene (rs2296651, rs61745930, and rs4646287) and assessed the risk of HBV infection among the Ghanaian population
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