Abstract
Introduction: Detection of dengue virus infection as soon as possible is critical for management of dengue virus infected patients. Immuno-chromatographic (ICT) tests are easy, cost effective method for dengue virus antigen detection.The sensitivity and specificity of ICT should compare with a gold standard test like RT-PCR. Aim of this study was to compare two test methods (ICT and RT-PCR), observe dengue serotype and seasonal impact on dengue infection.Methodology & result: The patients of Ibn Sina Medical College Hospital from October 2015 to October 2017 were tested for dengue NS1 antigen by ICT method. Out of 3201 sample tested 32.39% were found positive and 89 of which were re-tested for RT-PCR for comparison. Eighty eight of 89 NS1 positive cases showed positive by RT-PCR method giving an accuracy of 98.87%. Among the RT-PCR positive cases 45 were further analyzed for serotype. DEN-1, DEN-2 or both DEN- 1 and DEN-2 were found in 21, 23 and 1cases respectively. No cases of DEN-3 or DEN-4 were detected.Conclusion: This study showed that easily available and cost effective dengue NS1 antigen detection method (ICT) is as effective as molecular test (RT-PCR). DEN-1 and DEN-2 serotype were prevalent during last few years in Bangladesh. Continuous monitoring of dengue virus serotype is important for prevention and control of sudden epidemic by other serotype. Alert to be more during post monsoon when the peak of dengue virus infection was observed.Bangladesh Journal of Medical Science Vol.17(4) 2018 p.669-673
Highlights
Detection of dengue virus infection as soon as possible is critical for management of dengue virus infected patients
A real-time one-step reverse transcriptase PCR (RT-PCR) was performed using two sets of consensus primers, one primer set targeting a region of the nonstructural protein 5 (NS5) genesto detect all flaviviruses and the other primer set targeting a region of the capsid gene to detect all DEN serotypes
Result: Table-1 showed that total of 3201 samples from suspected dengue cases were tested for Dengue NS1 antigen
Summary
The incidence of dengue has increased dramatically around the world in last few decades. Increase housing in slum, overcrowding, and deterioration in waste management systems, intimately associated with unplanned urbanization All these factors created ideal conditions for increased transmission of mosquito-borne diseases.[2] Dengue infection is associated with climate variation. 11,12 In Ibn Sina Medical College hospital, for detection of NS1 antigen, commercially available rapid ImmunoChromatographic Test (ICT) was used for the patients who came within 5 to 7 days after onset of fever. Methodology: In Ibn Sina Medical College hospital, 3201 human serum samples of suspected dengue cases were tested who had history of fever for 1–7 days from October 2015 to October 2017. The Figure-1 showed the monthly distribution of suspected positive and negative dengue cases came to Ibn Sina Medical College hospital for testing
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