Abstract
Curled dock (Rumex crispus L.) is known to have several health benefits including antioxidant, antifungal, and anti-inflammatory effects. The potential to induce phase 2 detoxifying enzymes of butanol extract of curled dock (BECD) seeds was investigated. BECD increased the activity of NAD(P)H:quinone oxidoreductase and expression of phase 2 detoxifying enzymes such as heme oxygenase 1, Γ-glutamylcysteine synthetase, NAD(P)H:quinone oxidoreductase, and glutathione reductase, in both hepa1c1c7 and its mutant BPRc1 cells. The translocation of transcription factor nuclear factor-erythroid 2 p45-related factor 2 (Nrf2) and binding to antioxidant response element (ARE) in the promoter region of phase 2 detoxifying/antioxidant enzymes were promoted by BECD in HepG2-C8 cells. Furthermore, BECD enhanced phosphorylation of Akt in hepatoma cell lines while Nrf2 activation induced by BECD was suppressed by an inhibitor of Akt. This result suggests that BECD induces phase 2 detoxifying enzymes through the Akt/Nrf2/ARE signaling pathway. BECD merits further study to be developed as detoxification agent.
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