Abstract
NPR3 Inhibits EMT and Tumour Metastasis of Human Osteosarcoma by Activating AKT/mTOR-Mediated Autophagy
Highlights
Osteosarcoma is an aggressive primary malignant cancer of bone mainly occurring in adolescence with a characteristic of high metastasis and relapse rate
We found that over-expression of Natriuretic Peptide Receptor 3 (NPR3) could induce autophagy, promote apoptosis and inhibit EMT
Loss-of-function experiments displayed that effects of NPR3 were weakened by treatment with the specific autophagy inhibitor Baf-A1 and CQ
Summary
The expression of NPR3 in OS patients and cells was detected by qRT-PCR, and IHC analysis. A total of 122 fresh primary bone tumour tissues with metastases and 172 bone tumour tissues without distance tissues metastases of OS patients who underwent surgery were collected from Yunnan Cancer Hospital, Kunming, China (December 2013 to December 2020). These tissues were stored at − 80°C immediately after surgery. Paraffin sections of 19 OS cases with and without lung metastases were obtained from the Department of Pathology of the Yunnan Cancer Hospital, Kunming, China (May 2019 to December 2020) including the primary bone tumour tissues, para-cancer tissues. This study was approved by the Ethics Committee of Yunnan Cancer Hospital
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