NP and NS1 proteins of H5N1 virus significantly upregulated IFITM1, IFITM2, and IFITM3 in A549 cells.

Haifeng Wang,Lin Chen,Hongxuan He,Jing Luo

doi:10.4314/ahs.v19i1.13

Haifeng Wang, Lin Chen + Show 2 more

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https://doi.org/10.4314/ahs.v19i1.13

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Abstract

Avian influence virus H5N1 causes serious public health concern with significant morbidity and mortality from poultry to humans. Interferon-induced transmembrane (IFITM) proteins usually protect cells from many virus infections by viral entry and replication. The purpose of this study was to investigate whether H5N1 viral proteins involved in regulation IFITM1, IFITM2, and IFITM3 following H5N1 infection. NS1, M1, NP, PB2, HA and NA genes of H5N1 virus were generated by PCR and cloned into pcDNA3.1/myc-His (+) A vector for genes over-expression experiments. Gene expression levels was performed using Real-time PCR. Research displayed that NS1, M1, NP, and PB2 proteins of H5N1 virus increased IFITM1, IFITM2, and IFITM3 expression in A549 cells, only IFITM1 was upregulated by M1 in HEK293T cells. However, our study did not find that HA and NA of H5N1 virus affected IFITM genes family or interferon genes expression. Taken together, our data suggested that IFITM1, IFITM2, and IFITM3 might be directly upregulated via NS1, M1, NP, and PB2 proteins during H5N1 avian influenza virus infection. This study provided new insights into the influence of NS1 and NP proteins on regulation of IFITM1, IFITM2, and IFITM3 expression following H5N1 infection.

Highlights

Avian influenza A virus H5N1 is one of the greatest world-African Health SciencesAfrican Health Sciences Vol 19 Issue 1, March, 2019 brane proteins including IFITM1, IFITM2, IFITM3, IFITM5, and IFITM10
H5N1 promoted expression of IFITM1, 2, and 3 genes in A549 cells H5N1 virus induced death of A549 and HEK293T cells at 0.5, 1, 2 multiplicities of infection (MOI) for 1h, IFITM1, IFITM2, and IFITM3 expression increased in A549 cells, only IFITM2 and IF
IFNA1, IFNB1, and IFNG were upregulated by H5N1 infection Our study demonstrated that H5N1 avian influenza virus could upregulate IFNA1, IFNB1, and IFNG in both A549 and HEK293T cells (Figure 3)

Summary

Introduction

Avian influenza A virus H5N1 is one of the greatest world-African Health SciencesAfrican Health Sciences Vol 19 Issue 1, March, 2019 brane proteins including IFITM1, IFITM2, IFITM3, IFITM5, and IFITM10. Ubiquitination and deubiquitination of NP protein regulated influenza A virus RNA replication[9]. Interferon-induced transmembrane (IFITM) proteins usually protect cells from many virus infections by viral entry and replication. Objectives: The purpose of this study was to investigate whether H5N1 viral proteins involved in regulation IFITM1, IFITM2, and IFITM3 following H5N1 infection. Our study did not find that HA and NA of H5N1 virus affected IFITM genes family or interferon genes expression. Conclusion: Taken together, our data suggested that IFITM1, IFITM2, and IFITM3 might be directly upregulated via NS1, M1, NP, and PB2 proteins during H5N1 avian influenza virus infection. This study provided new insights into the influence of NS1 and NP proteins on regulation of IFITM1, IFITM2, and IFITM3 expression following H5N1 infection.

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