Abstract
The recovery of single circulating tumor cells (CTCs) from the peripheral blood of cancer patients has great potential for the study of cell heterogeneity and cancer metastasis and the development of personalized cancer immunotherapy. Here we present nozzleless droplet generation with focused acoustic beams for cell encapsulation. The mechanism of droplet generation is sensitive to the pulse width and the droplet diameter ranges from 350 to 550 μm. The pulse width duration (520 μs) and cell concentration (5 × 103 cells ml−1) can be adjusted to obtain the maximum probability (11.61%) of single cell encapsulation. Three-color fluorescence is used to identify encapsulated cells in the droplet and target cells are extracted by microcapillarity for conducting single cell analysis. The reported method of using acoustic tweezers to eject the droplet has advantages of convenience, speed and biocompatibility while being non-invasive, and could become a powerful tool for encapsulating single CTCs.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
