Abstract
ABSTRACT: Plant viruses have a wide range of weed/wild hosts, which can act as virus reservoirs. Centosema brasilianum is a wild leguminous species frequently found in association with cultivated fields in the Northeast region of the country. In March 2017, a plant of C. brasilianum was found showing symptoms of mosaic and leaf rolling, in the municipality of Maceio, state of Alagoas. In this context, the present study aimed to detect and molecular characterize viruses with DNA genomes infecting C. brasilianum. Total DNA was extracted from the leaf sample and used as a template via PCR amplification reactions assays using universal primers, aiming respectively at the detection of badnavirus and begomovirus. Partial sequences were obtained for the badnavirus RT/RNaseH and begomovirus DNA-A regions. Comparisons of paired sequences and phylogenetic analyzes indicated the presence of coinfection in C. brasilianum by badnavirus and begomovirus. The isolate BR:Mac:17 should be considered a putative new species of badnavirus, for which the name ‘Centrosema bacilliform virus’ (CenBV) is proposed. This species was phylogenetically more related to Dioscorea bacilliform AL virus (DBALV, KX008573), from the Dioscorea rotundata. Meanwhile, the isolate BR:Mac2:17 is a Begomovirus, probably of the species Bean golden mosaic virus (BGMV). The results demonstrate that C. brasilianum can act as a reservoir of badnavirus and begomovirus in the absence of culture in the field. KEYWORDS: Molecular characterization, Caulimoviridae, Geminiviridae, non-cultivated plants.
Highlights
NOVO BADNAVÍRUS EM INFECÇÃO MISTA COM BEGOMOVÍRUS EM Centrosema brasilianum NEW BADNAVIRUS IN MIXED INFECTION WITH BEGOMOVIRUS IN Centrosema brasilianum TEIXEIRA, Raíza da Rocha Oliveira; MELO, Aline Marques; FERRO, Mayra Machado de Medeiros; SILVA, Sarah
Centosema brasilianum is a wild leguminous species frequently found in association with cultivated fields in the Northeast region of the country
Total DNA was extracted from the leaf sample and used as a template via PCR amplification reactions assays using universal primers, aiming respectively at the detection of badnavirus and begomovirus
Summary
New badnavirus in mixed infection with begomovirus in Centrosema brasilianum Raíza da Rocha Oliveira Teixeira ;(1) Aline Marques Melo ;(2) Mayra Machado de Medeiros Ferro ;(3) Sarah Jacqueline Cavalcanti da Silva ;(4). Centosema brasilianum é uma espécie silvestre leguminosa frequentemente encontrada em associação com campos de cultivo na região Nordeste do país. Em março de 2017, foi encontrada uma planta de C. brasilianum apresentando sintomas de mosaico e enrolamento foliar, no município de Maceió, estado de Alagoas. O presente estudo teve como objetivo detectar e caracterizar molecularmente vírus com genoma de DNA infectando C. brasilianum. O DNA total foi extraído a partir da amostra foliar e usado como molde para reações de amplificação via PCR utilizando os primers universais para detecção de badnavírus e begomovírus. O isolado BR:Mac: pode ser considerado uma putativa nova espécie de badnavírus para a qual o nome ‘Centrosema bacilliform virus’ (CenBV) foi proposto. Os resultados demonstram que C. brasilianum pode atuar como reservatório de badnavírus e begomovírus na ausência da cultura no campo.
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