Abstract
A selective, precise, and accurate high-performance thin-layer chromatographic (HPTLC) method has been developed for the analysis of Nagod samples and its formulations for their betulinic acid content. The method involves densitometric evaluation of betulinic acid after resolving it by HPTLC on silica gel plates with toluene—acetone—formic acid (17:3:0.2, v/v/v) as the mobile phase. For densitometric evaluation, peak areas were recorded at 445 nm after derivatizing the resolved bands with anisaldehyde—sulphuric acid reagent. The relation between the concentration of betulinic acid and the corresponding peak areas was found to be linear within the range of 200–1200 ng/spot. The method was validated for linearity range, precision, limit of detection (LOD), limit of quantification (LOQ), and accuracy and was demonstrated for quantification of betulinic acid in Vitex negundo L. plant parts and its commercial polyherbal formulations. The method was able to identify and quantify betulinic acid in complex mixtur...
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