Abstract
Esterases are important enzymes because of their ability to catalyze a number of hydrolytic reactions. Cloning and overexpression of esterase genes provides a useful strategy to make further esterases available for technical use. A genomic library of Rhodococcus sp. NCIMB 11216 was screened by activity assay using α-naphthol-acetate. Two independent esterase genes were isolated and one of them showed ability to hydrolyse esters of tertiary alcohols. By rescreening the genomic library using the bulky substrate, naphthol AS-D acetate, a few further esterase genes were isolated.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Published version (
Free)
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
